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Dependence of v/[E] for decarboxylation of FOMP catalyzed by mutant forms of ScOMPDC on the concentration of FOMP for reactions at 25 °C, pH 7.1 (30 mM MOPS), and I = 0.105 (NaCl): (A) (▲) Y217F mutant, (●) Q215A/Y217F mutant, and (inset) Q215A mutant; (B) (▲) Q215A/R235A mutant and (●) Y217F/R235A mutant; and (C) (●) S154A mutant and (○) Q215A/S154A mutant.
