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MicroRNA-9 (miR-9) inhibits human colorectal cancer cell proliferation and growth. (a, b) After transfection with pre-miR-9 and pre-miR-control (pre-miR-cont) for 72 h in HCT116 and HT29 cells, miR-9 expression was detected by RT-PCR. (c, d) Upregulation of miR-9 repressed cell proliferation as determined by CCK-8 assays. (e, f) Colony formation was carried out to analyze cell proliferation. $P < 0.05. OD, optical density.
