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. 2015 May 26;106(7):848–856. doi: 10.1111/cas.12680

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© 2015 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Fig 1 — In vivo and in vitro SUMO modification of NACC1. (a) In vivo SUMOylation of nucleus accumbens associated 1 (NACC1) in HeLa cells. (b) Analysis of SUMO modification by reconstituted in vitro SUMOylation reactions. Slow-migrating bands due to addition of SUMO paralogues were observed. (c) Confocal immunofluorescence of endogenous NACC1 and SUMO1 proteins in HeLa cells (NACC1, green; SUMO1, red). Both NACC1 and SUMO1 were observed in nucleus and cytoplasm with a granular pattern. Bar, 10 μm. (d) Colocalization of GFP-NACC1 and Cherry-SUMO paralogues (NACC1, green; SUMO1, red). Bar, 10 μm.