Fig 4.

Histone deacetylase 3 (HDAC3) influences vasculogenic mimicry (VM) in U87MG cells. (a) U87MG cells were transfected with siRNAs and HDAC3 mRNA levels were detected by quantitative RT-PCR. Gene expression was normalized to that of the housekeeping gene 18S rRNA. Data are presented as fold induction relative to the expression of the U87MG group and are represented as mean ± SD ($P < 0.05). (b) Western blot and densitometry analysis of HDAC3 protein between groups transfected with siRNA ($P < 0.05). (c) U87MG cells subjected to different treatments were seeded into wells of a 24-well plate coated with Matrigel for 6 h then photographed. Scale bar = 100 μm. (d) Total length of VM tubes and the number of intersections per field were compared between groups in (c) ($P < 0.05 compared with U87MG).