Table 2.
Primer sequences used for quantitative RT-PCR
| Gene | Primer sequence (5′ to 3′) | Size, bp |
|---|---|---|
| HDAC3 | F: CCCTGCGGGATGGCATTGATGA | 123 |
| R: AGCCCAGAGAGTCAGCTCCACA | ||
| MMP-2 | F: GGCGGTCACAGCTACTTCTTC | 105 |
| R: GCAGCCTAGCCAGTCGGATT | ||
| MMP-9 | F: GTGACACCGCTCACCTTCAC | 122 |
| R: GCGTGTGCCAGTAGACCATC | ||
| MMP-14 | F: CTGCGTCCATCAACACTGCCTA | 128 |
| R: GCCCAGCTCCTTAATGTGCTTG | ||
| EphA2 | F: TTAGGGAGAAGGATGGTGAGTT | 140 |
| R: GGTCGAGGGCATGGTGTA | ||
| VE-cadherin | F: TTTCCAGCAGCCTTTCTACCA | 145 |
| R: GGAAGAACTGGCCCTTGTCA | ||
| LAMC2 | F: TCGGGAGCCATGTCATGTGAGTG | 148 |
| R: CCCAGCATCAGGAAGCAAGGAGT | ||
| hu-18S | F: GACTCAACACGGGAAACCTCAC | 122 |
| R: CCAGACAAATCGCTCCACCAAC |
EphA2, ephrin type-A receptor 2; F, forward; LAMC2, laminin, γ2; R, reverse; VE, vascular endothelial.