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. 2015 May 26;106(7):875–882. doi: 10.1111/cas.12686

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© 2015 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Fig 2 — Effects of platelet-derived growth factor-BB (PDGF-BB) on AXT osteosarcoma cell proliferation and migration. (a) Cell proliferation assay with AXT cells in the presence of the indicated concentrations of PDGF-BB in the absence (left) or presence (right) of FBS. Data are expressed relative to the value for time 0. $P < 0.05. NS, not significant. (b) Migration assay with AXT cells in the absence or presence of PDGF-BB. (c) Immunoblot analysis of AXT cells deprived of serum for 2 h and then stimulated with PDGF-BB for 15 min in serum-free or FBS medium. PDGF receptor β (PDGFRβ) was immunoprecipitated (IP) from cell lysates before immunoblot analysis.