Fig 4. Three consecutive regions within the inhibitory core exhibit coordinated suppression of myostatin activity.

Constructs (ΔRC, ΔAH, and ΔLL) with single deletions of the RC, AH, or LL regions of full-length myostatin (f-Pro) showed an inhibitory effect on the transcriptional activities of myostatin (A) and GDF11 (B), but not activin A (C) or TGF-β1 (D), in HEK293 cells. In contrast, combined deletion constructs (ΔRCΔAH, ΔAHΔLL, and ΔRCΔAHΔLL) had no inhibitory effect on myostatin (A) or GDF11 (B) activation in the HEK293-(CAGA)₁₂-luciferase system.