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. Author manuscript; available in PMC: 2016 Jul 21.
Published in final edited form as: Cell Rep. 2015 Jul 9;12(3):449–461. doi: 10.1016/j.celrep.2015.06.023

Figure 1. SK2 expression in hippocampus of AS and WT mice.

Figure 1

(A,B) Western blot analysis of SK2 levels in (A) crude synaptosomal fractions (P2) and (B) PSD fractions (P3). Optical densities of the SK2 band are normalized to those of β-actin or PSD-95. Data are expressed as % of values in WT mice and shown as means ± S.E.M of 9-10 (in a) or 3 mice (in b) from at least 3 litters, ***p <0.001 (unpaired t-test) and **p < 0.01 (unpaired t-test).

(C) Localization of synaptic SK2 in AS mice. Representative images of CA1 pyramidal neurons stained with anti-SK2 (red) and -PSD95 (green) antibodies. Arrowheads indicate clearly co-localized puncta. Left panel, scale bar = 20 μm. Right panel, scale bar = 10 μm.

(D) Quantitative analysis of the number of SK2-immunoreactive puncta and percentage of SK2 and PSD95 dually stained puncta/synapses in hippocampal CA1 region. N = 18-24 regions of interest from at least 9 slices from at least 3 mice per group (unpaired t-test, ***p<0.001). See also Figure S1.

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