Figure 7. Loss of Rbpj dependent hypothalamic Notch signaling does not affect pituitary hormone cells at e16.5.
Hematoxylin and eosin (H&E) staining reveals anterior lobe (AL), intermediate lobe (IL) and posterior lobe (PL) morphology in coronal pituitary sections of control (A) and Rbpj cKO (B) mice. Luteinizing hormone (LH) is not readily detectable in either control (Fig. 7C) or Rbpj cKO pituitaries (Fig. 7D) at e16.5. In control pituitaries, growth hormone (GH; E), thyroid stimulating hormone (TSH; G), proopiomelanocortin (POMC; I) are specified by e16.5. RbpjcKO pituitaries also display specification of these hormone producing cells (F, H, J). Scale bar, 50μm.