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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Neurogastroenterol Motil. 2015 Jun 18;27(8):1162–1174. doi: 10.1111/nmo.12604

Figure 2.

Figure 2

Expression of BDNF protein and mRNA in smooth muscle cells of rabbit intestine. (A) Western blot of BDNF protein in homogenates of longitudinal and circular muscle cells. Insert illustrates a typical Western blot of BDNF and β actin. Bar graph illustrates optical density of BDNF blots normalized to β actin. Values are Mean ± SEM; n=8; **= p<0.01 for difference between circular and longitudinal muscle. (B) Quantative rt-PCR of BDNF mRNA in circular and longitudinal muscle cells. Relative expression was calculated by the ΔΔCT method as described in Methods. Values are Mean± SEM; n=5; **= p<0.01 for difference between circular and longitudinal muscle.