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Secretion of BDNF induced by PACAP and substance P. Incubation of longitudinal smooth muscle cells in culture with PACAP at 10 and 100 nM for 24 (A) and 48 (B) hrs and with substance P at 10 and 100 nM for 24 (C) and 48 (D) hrs increased secretion of BDNF above control levels obtained with incubation in DMEM-0. BDNF in the culture medium was measured by specific ELISA. Values are Mean ± SEM; n=3–5; **= p<0.01 for difference from expression in presence of DMEM-0.
