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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Neurogastroenterol Motil. 2015 Jun 18;27(8):1162–1174. doi: 10.1111/nmo.12604

Figure 5.

Figure 5

Effect of BAPTA on the expression and secretion of BDNF induced by substance P. Incubation of smooth muscle cells in cultures for 24 hours with the Ca2+ chelating agent BAPTA abolished the increase in BDNF expression (A) and secretion (B) induced by 10 nM substance P. In Western blots (A), optical density of BDNF blots was normalized to β actin. Inserts illustrate typical blot. BDNF secretion (B) was measured in the culture medium by specific ELISA. Values are Mean ± SEM; n=3–5; **= p<0.01 for difference from expression in presence of DMEM-0.