Figure 6.
Effect of BAPTA on BDNF mRNA levels induced by substance P. Incubation of smooth muscle cells for 24 hours with the Ca2+ chelating agent BAPTA abolished the increase in BDNF mRNA levels induced by 10 nM substance P. Relative expression was calculated by the ΔΔCT method as described in Methods. Values are Mean ± SEM; n=3–5; **= p<0.01 for difference from expression in presence of DMEM-0.