Inter-domain salt bridges between MII and MIII subdomains after the MII/MIII reorientation during the cf-SMD simulations (trajectories 29 and 30). Salt bridges are shown in the last conformation after 325-ns cf-SMD simulations, cf-SMD1 (A) and cf-SMD2 (B). The second helix in the MIII subdomain is a conserved site with several charged residues (Arg-540, Arg-546, Arg-551, and Asp-561), which form salt bridges with the MII subdomain.