FIGURE 9.

Inconsistency of conformational changes during cv-SMD simulations for the MI–MIII fragment. Six cv-SMD simulations were performed at different velocities: cv-SMD1, cv-SMD2, cv-SMD3, and cv-SMD4 were pulled with a constant velocity of 10 Å/ns, although the velocity in cv-SMD5 and cv-SMD6 is 2 Å/ns. Backbone r.m.s.d. of each domain, and the vinculin-binding site (the 2nd and 3rd α-helices (MI h23)), are measured to show different patterns of conformational changes. The force-time profile and final conformation are shown for each cv-SMD.