FIGURE 8.

Effect of the I315G substitution on HCV RNA replication. NS3–5B subgenomic Con1 replicons containing cell culture adaptive mutations (E1202G, T1280I, and K1846T) designated ET (45) and additional mutations I315G or P314A in NS5A or a defective polymerase mutant (GND) were electroporated into Huh-7 cells. The cells were lysed at the given time points, and the luciferase activities were determined. The data were normalized to the respective 4-h value reflecting transfection efficiency. Mean values of quadruplicate measurements of two independent experiments are shown. The error bars indicate standard deviations. The horizontal dashed line indicates the background.