FIGURE 3.

AICAR treatment increases AMPK phosphorylation and decreases Tau phosphorylation. A, HK-532 cells were differentiated for 1 week and treated with 1 mm AICAR for 0–6 h (left panel) or increasing concentrations of AICAR for 2 h (middle panel). The results of AMPK phosphorylation with 1 mm AICAR for 2 h treatment are shown in the graph on the right. B, HK-532 cells were treated with 1 mm AICAR for 2 h, and Tau phosphorylation was examined. C, primary rat embryonic cortical neurons were treated with 1 mm AICAR for 0–6 h. Cell lysates were prepared in radioimmune precipitation assay buffer and immunoblotted with the indicated antibodies, Anti-actin immunoblots demonstrate the equal protein loading. *, p < 0.05 by t test.