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. 2015 Jun 22;290(31):19146–19157. doi: 10.1074/jbc.M115.636852

FIGURE 4.

FIGURE 4.

Insulin pretreatment specifically increases AMPKSer-485 phosphorylation and prevents AICAR-induced Tau dephosphorylation. A, HK-532 cells were treated without (− Ins) or with (+ Ins) 50 nm insulin overnight and then treated with 1 mm AICAR for 0, 2, or 6 h. B, when the cells were pretreated with 50 nm insulin overnight, AICAR treatment specifically increased AMPKSer-485 phosphorylation. *, p < 0.05 compared with Ser-485 phosphorylation without insulin pretreatment at same time point. C, the cells were treated without or with 50 nm insulin overnight and then treated with 1 mm AICAR for 2 h. Insulin pretreatment prevented AICAR-induced Tau dephosphorylation. D, densitometric analysis of phosphorylated Tau (pTau). E, insulin pretreatment also prevented AICAR-induced Tau dephosphorylation in embryonic rat primary cortical neurons (eCN). *, p < 0.05 compared with control by t test.