Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Protein expression, purification, and immunoblotting analysis of WT and its mutants. A, SDS-PAGE analysis of purified WT and its mutants, after isopropyl β-d-thiogalactoside-induced expression for 6 h. The proteins were extracted with 2% SDS, followed with 3% n-octylpolyoxyethylene, and further purified using Hi-Trap Q column chromatography. Immunoblotting detected with anti-VhChiP (B) and anti-OmpN (C) polyclonal antibodies.
