TABLE 1.

Primers for site-directed mutagenesis

Underlined sequences indicate the mutated codons.

Mutation	Nucleotide sequence
Trp136 (WT)
Forward	5′-ataccatggcgtcttacctaaagaaaag-3′ (NcoI)
Reverse	5′-aacctcgagttagaagtagtattcaacac-3′ (XhoI)
Trp136 → Ala
Forward	5′-ggtctaggtgatgtttacgacgcaggtggtgctatcggtggtgc-3′
Reverse	5′-gcaccaccgatagcaccacctgcgtcgtaaacatcacctagacc-3′
Trp136 → Phe
Forward	5′-ggtctaggcgatgtttacgactttggtggtgcgattggtggtgc-3′
Reverse	5′-gcaccaccaatcgcaccaccaaagtcgtaaacatcgcctagacc-3′
Trp136 → Asp
Forward	5′-ggtctaggcgatgtttacgacgatggtggtgcgatctgtggtgc-3′
Reverse	5′-gcaccacagatcgcaccaccatcgtcgtaaacatcgcctagacc-3′
Trp136 → Arg
Forward	5′-ggtctaggcgatgtttacgaccgtggtggtgcgatctgtggtgc-3′
Reverse	5′-gcaccacagatcgcaccaccacggtcgtaaacatcgcctagacc-3′