TABLE 2.

Single channel conductance of VhChiP wild-type and mutants, as determined by two different BLM techniques

The membranes were formed by dissolving 1,2-diphytanoyl-sn-glycero-3-phosphatidylcholine in n-pentane or n-decane. The electrolyte contained 1 m KCl in 20 mm HEPES, pH 7.5. The applied potential was −100 mV, and the BLM measurements were carried at 20 ± 3 °C. The three conductance values shown in the solvent-containing membrane BLM technique correspond to the opening of a trimeric channel. The biggest values present the opening of three channels, whereas the two-thirds and one-third values were related to two and one openings, respectively. n shown in parentheses represents number of channels that were used for calculating ion conductance.

1 m KCl (20 mm HEPES, pH 7.5)	Conductance (G)
	Wild type	W136A	W136F	W136D	W136R
	nS
Solvent-depleted membrane	1.9 ± 0.03 (17)	2.2 ± 0.05 (16)	1.9 ± 0.02 (18)	2.3 ± 0.03 (22)	1.7 ± 0.02 (12)
Solvent-containing membrane	1.7 ± 0.30 (79)	2.5 ± 0.33 (80)	1.8 ± 0.33 (27)	2.4 ± 0.52 (22)	1.6 ± 0.35 (45)
	1.1 ± 0.08 (101)	1.5 ± 0.23 (116)	1.2 ± 0.22 (48)
	0.6 ± 0.17 (238)	0.8 ± 0.27 (230)	0.6 ± 0.21 (150)	0.8 ± 0.43 (206)	0.6 ± 0.25 (52)