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Determination of the equilibrium binding constant (K) of VhChiP WT and its mutants with chitohexaose from the non-linear curve fit shown in Fig. 8, following Equation 5
| Ligand | VhChiP variant | K |
|---|---|---|
| m−1 | ||
| Chitohexaose | WT | 11,000,000 ± 350,000 |
| W136A | 909,000 ± 16,500 | |
| W136F | 4,000,000 ± 130,000 | |
| W136D | 530,000 ± 20,000 | |
| W136R | 1,150,000 ± 250,000 |
