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Production of PNAG in alginate-deficient P. aeruginosa FRD1 in the presence of the B. subtilis epsHIJK locus. WT P. aeruginosa FRD1 produces alginate and binds Alexa Fluor 48-labeled mAb F429 but not PNAG-specific mAb F598. A polar Tn-insertion in the algF gene eliminated alginate production and insertion of the B. subtilis epsHIJK locus leads to PNAG production. Immunoreactive PNAG in this latter strain is lost after treatment with dispersin B or periodate. White bars, 10 μm.
