TABLE 3.

Summary for detection of PNAG synthesis by immunofluorescence in indicated strain

The following symbols are used: −, no immunofluorescence detected; +, 25–50% of WT levels of PNAG observed; ++, 51–80% of WT levels of PNAG observed; +++, >80% of WT levels of PNAG observed.

Strain	Complemented with	Control mAb	mAb to PNAG
E. coli K1 WT		−	+++
E. coli K1 ΔpgaA		−	−
E. coli K1 ΔpgaB		−	−
E. coli K1 ΔpgaC		−	−
E. coli K1 ΔpgaD		−	−
E. coli K1 ΔpgaA	(pUCP18Tc)	−	−
E. coli K1 ΔpgaB		−	−
E. coli K1 ΔpgaC		−	−
E. coli K1 ΔpgaD		−	−
E. coli K1 ΔpgaA	(pUCP18Tc-pgaA)	−	+++
E. coli K1 ΔpgaB	(pUCP18Tc-pgaB)	−	+++
E. coli K1 ΔpgaC	(pUCP18Tc-pgaC)	−	+++
E. coli K1 ΔpgaD	(pUCP18Tc-pgaD)	−	+++
E. coli K1 ΔpgaA	(pUCP18Tc-epsH)	−	+++
E. coli K1 ΔpgaA	(pUCP18Tc-epsJ)	−	+
E. coli K1 ΔpgaB	(pUCP18Tc-epsI)	−	+++
E. coli K1 ΔpgaC	(pUCP18Tc-epsH)	−	+
E. coli K1 ΔpgaC	(pUCP18Tc-epsJ)	−	+++
E. coli K1 ΔpgaD	(pUCP18Tc-epsK)	−	+++
E. coli K1 ΔpgaABCD	(pUCP18Tc-epsHIJK)	−	+++
K. pneumoniae Δpga	(pUCP18Tc)	−	−
K. pneumoniae Δpga	(pUCP18Tc-epsHIJK)	−	+++