Skip to main content
. 2015 May 29;290(31):19287–19306. doi: 10.1074/jbc.M114.623561

FIGURE 6.

FIGURE 6.

Western blot analysis of ROSA stop transgenic mice. A, Western blot analysis of 4.5-month-old lysates from N171-Q148, N463-Q148, N536-Q148, N552-Q148, and N586-Q148 transgenic ROSA HD transgenic mouse models using distinct HTT antibodies to distinct regions of the protein. The open arrowhead indicates where the cleavage product should be detected. The product is absent. B, schematic of the proteolysis or fragments of HTT present in cortical lysates of the transgenic mouse models. The lower Western blot demonstrates the appearance of C-terminal cleavage fragments detected with HTT MAB2166.