TABLE 1.
SiR proteins and their expression plasmids
| Protein | Expression plasmid | Strain | Tag | Oligomeric state |
|---|---|---|---|---|
| His6-SiRHP | pBAD/myc/his A | LMG194 | N-terminal His6 | Monomer |
| SiRHP | pBAD/myc A | LMG194 | N-terminal His6 | Monomer |
| Δ60-SiRHP | pBAD/myc A | LMG194 | N-terminal His6 | Monomer |
| Δ80-SiRHP | pBAD/myc A | LMG194 | N-terminal His6 | Monomer |
| Apo-wild type SiRHP | pBAD/myc/his A | JW3331 (cVsG−) | N-terminal His6 | Tetramer |
| Apo-Δ60-SiRHP | pBAD/myc/his A | JW3331 (cVsG−) | C-terminal His6 | Monomer |
| Apo-Δ80-SiRHP | pBAD/myc/his A | JW3331 (cVsG−) | C-terminal His6 | Monomer |
| SiRFP | pCDFDuet-1 | BL21(DE3) | Octomer | |
| SiRFPFMN | pCDFDuet-1 | BL21(DE3) | N-terminal His6 | Octomer |
| SiRFP43 | pET-HTG | BL21(DE3) | N-terminal His6-GST/cleaved | Monomer |
| SiR | pBAD/myc/his A | LMG194 | N-terminal His6 (SiRFP) | Octomer/Tetramer |