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. 2015 Jun 19;290(31):19334–19342. doi: 10.1074/jbc.M115.668343

TABLE 2.

MS/MS fragments of the tryptic peptide containing the H258F-F5 mutation

The full peptide sequence is 226AKTDS NKDNVYVNFL SVASGGSAFN STYNYASF-F5IN PEIAK265, and the MS/MS data are shown in Fig. 1.

C-terminal fragment Fragment sequence Predicted mass Measured mass
Da Da
y5+ 261PEIAK265 557.33 557.33
y6+ 260N PEIAK265 671.37 671.40
y7+ 259IN PEIAK265 784.46 784.47
y8+ 258F-F5IN PEIAK265 1021.48 1021.47
y9+ 257SF-F5IN PEIAK265 1108.51 1108.60
y10+ 256ASF-F5IN PEIAK265 1179.55 1179.60
y11+ 255YASF-F5IN PEIAK265 1342.61 1342.60
y12+ 254NYASF-F5IN PEIAK265 1456.65 1456.60
y13+ 253YNYASF-F5IN PEIAK265 1619.72 1619.73
y15+ 251STYNYASF-F5IN PEIAK265 1807.80 1807.40
y16+ 250N STYNYASF-F5IN PEIAK265 1921.84 1921.40