Fig. 3.

Poly I:C-induced systemic and CNS synthesis of inflammatory cytokines. ELISA analysis of plasma IL-6, TNF-α, IL-1β and IFN-β (a-d) and CNS cytokine transcription 3 h after intraperitoneal challenge with poly I:C (12 mg/kg). Analysis of transcripts is shown for hypothalamus (e–h) and hippocampus (i–l). Data are expressed as mean ± SEM, n = 5 for WT and n = 6 for IFNAR1^−/−. Comparisons of pI:C treatment for WT and IFNAR1^−/− mice were carried out by two-way ANOVA, with treatment and strain as factors. Main effects of treatment are denoted by ^###p < 0.001, ^##p < 0.01 and ^#p < 0.05 and interactions between treatment and strain are denoted by ^∗∗∗p < 0.001 and ^∗∗p < 0.001.