Corrected t-test sample size calculations; α = 0.01
Group 1	Group 2	Effect size d	Power	Sample size per group
10 mM D-2-HG	10 mM L-2-HG	65.25231	99.9%	2
50 mM D-2-HG	50 mM L-2-HG	57.34623	99.9%	2
10 mM D-2-HG	50 mM D-2-HG	51.14839	99.9%	2

Corrected t-test sample size calculations; α = 0.01
Group 1:	Constant	Effect size d	Power	Sample size per group
10 mM D-2-HG	1	24.62687	97.7%	2
50 mM D-2-HG	1	244.11765	99.9%	2