Corrected t-test sample size calculations; α = 0.01
Group 1	Group 2	Effect size d	Power	Sample size per group
10 mM D-2-HG	10 mM L-2-HG	8.70031	99.9%	3
50 mM D-2-HG	50 mM L-2-HG	7.64616	99.4%	3
10 mM D-2-HG	50 mM D-2-HG	6.81978	97.9%	3

Corrected t-test sample size calculations; α = 0.01
Group 1:	Constant	Effect size d	Power	Sample size per group
10 mM D-2-HG	1	3.28358	87.2%	6
50 mM D-2-HG	1	32.54902	99.5%	3