D-2-HG |
Reagent |
Sigma–Aldrich |
H8378 |
|
L-2-HG |
Reagent |
Sigma–Aldrich |
90790 |
|
Sf9 cells |
Cells |
ATCC |
CRL-1711 |
Original unspecified |
Shrimp alkaline phosphatase |
Reagent |
New England Biolabs |
MO371S |
|
T4 polynucleotide kinase |
Reagent |
Sigma–Aldrich |
KEM0006 |
|
DNase I |
Reagent |
Sigma–Aldrich |
AMPD1 |
|
Phosphodiesterase I |
Reagent |
Sigma–Aldrich |
P3243 |
|
PEI-cellulose TLC plate |
Material |
Sigma–Aldrich |
Z122882 |
|
FLAG-TET2-CD viral particles |
Virus |
Provided by the original authors |
Anti-Flag M2 antibody agarose affinity gel |
Reagent |
Sigma–Aldrich |
A2220 |
|
Flag peptide |
Reagent |
Sigma–Aldrich |
F4799 |
|
α-KG |
Reagent |
Sigma–Aldrich |
75,892 |
|
GenElute PCR Clean-Up Kit |
Kit |
Sigma–Aldrich |
NA1020-1KT |
Replaces Qiagen cat no. 28304 |
[γ-32]ATP |
Reagent |
Perkin Elmer |
BLU502H/NEG502H |
|
MspI methyltransferase |
Reagent |
NEB |
M0215L |
|
MspI restriction endonuclease |
Reagent |
NEB |
R0106T |
|
DNA duplex oligonucleotide substrate |
oligo |
Integrated DNA Technologies |
custom 5′-GTGTTCTTTCAGCTCCGGTCACGCTGACCAGC-3′ as a duplex oligo, HPLC purified at 1 umole scale maybe higher depending on recovery |
M13-F primer |
oligo |
Integrated DNA Technologies |
|
CCAGTCACGACGTTGTAAAACG |
M13-R primer |
oligo |
Integrated DNA Technologies |
|
CCAGTCACGACGTTGTAAAACG |
JumpStart REDTaq DNA Polymerase |
Reagent |
Sigma |
D8189-50UN |
|
dNTP mix 10 mM |
Reagent |
Sigma |
D7295-.2 ML |
|
BlueView TAE buffer |
Buffer |
Sigma |
T8935-1L |
|
Molecular biology grade water |
Reagent |
Sigma |
W4502-1L |
|