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. 2015 Jul 31;4:e06434. doi: 10.7554/eLife.06434
Reagent Type Manufacturer Catalog # Comments
LAPC4 cells Cells Provided by original authors
2 mm × 4 mm die set Instrument International Crystal Laboratories
NOD/SCID mice, male, 6–8 weeks old Mice Jackson Laboratories 001303
Ketamine HCl Reagent Jiang Su Heng Rui Medicine Co. H32022820 Replaces Cat. No. 10177 from Henry Schein Animal Health
Xylazine Reagent Huamu Animal Health Products Co. (2009) 070011582 Replaces Cat. No. 10177 from Henry Schein Animal Health
IsoFlo isoflurane Reagent Henry Schein Animal Health (Abbott) 017579 Optional
Betadine solution Reagent Henry Schein Animal Health (Purdue Frederick) 038248
Testosterone propionate powder Reagent Sigma–Aldrich T-1875
Flunixiject 50 mg/ml (banamine) Reagent Henry Schein Animal Health (Bimeda) 14165
Ethanol Reagent Specific brand information will be left to the discretion of the replicating lab and recorded later
Sterile saline Reagents Specific brand information will be left to the discretion of the replicating lab and recorded later
Matrigel matrix, high concentration Reagent BD 354248
IMDM with GlutaMAX, HEPES and sodium pyruvate Medium Gibco/Life Technologies 31980-030 This reagent was referred to as ‘DMEM’ in the original study
FBS Reagent Sigma–Aldrich F2442 Replaces Benchmark Cat. #100-106
Insulin needle and syringe Materials BD 320310