LAPC4 cells |
Cells |
Provided by original authors |
2 mm × 4 mm die set |
Instrument |
International Crystal Laboratories |
– |
– |
NOD/SCID mice, male, 6–8 weeks old |
Mice |
Jackson Laboratories |
001303 |
– |
Ketamine HCl |
Reagent |
Jiang Su Heng Rui Medicine Co. |
H32022820 |
Replaces Cat. No. 10177 from Henry Schein Animal Health |
Xylazine |
Reagent |
Huamu Animal Health Products Co. |
(2009) 070011582 |
Replaces Cat. No. 10177 from Henry Schein Animal Health |
IsoFlo isoflurane |
Reagent |
Henry Schein Animal Health (Abbott) |
017579 |
Optional |
Betadine solution |
Reagent |
Henry Schein Animal Health (Purdue Frederick) |
038248 |
– |
Testosterone propionate powder |
Reagent |
Sigma–Aldrich |
T-1875 |
– |
Flunixiject 50 mg/ml (banamine) |
Reagent |
Henry Schein Animal Health (Bimeda) |
14165 |
– |
Ethanol |
Reagent |
Specific brand information will be left to the discretion of the replicating lab and recorded later |
Sterile saline |
Reagents |
Specific brand information will be left to the discretion of the replicating lab and recorded later |
Matrigel matrix, high concentration |
Reagent |
BD |
354248 |
– |
IMDM with GlutaMAX, HEPES and sodium pyruvate |
Medium |
Gibco/Life Technologies |
31980-030 |
This reagent was referred to as ‘DMEM’ in the original study |
FBS |
Reagent |
Sigma–Aldrich |
F2442 |
Replaces Benchmark Cat. #100-106 |
Insulin needle and syringe |
Materials |
BD |
320310 |
– |