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. 2015 Jul 31;4:e06434. doi: 10.7554/eLife.06434
Reagent Type Manufacturer Catalog # Comments
FITC-conjugated monoclonal anti-CD44 (clone G44-26) Antibody BD Pharmingen 555478
FITC-mouse IgG2b isotype control immunoglobulin Antibody BD Pharmingen 555742
MX3005P Real-time PCR system Equipment Stratagene Replaces ABI Prism 7900 SDS
IMDM with GlutaMAX, HEPES and sodium pyruvate Medium Gibco/Life Technologies 31980-030 This reagent was referred to as ‘DMEM’ in the original study
miR-34a miRNA assay kit Primer Applied Biosystems TaqMan miRNA assay, assay ID 000426
hsa-let-7b miRNA assay kit Primer Applied Biosystems TaqMan miRNA assay, assay ID 000378
miR-191 miRNA assay kit Primer Applied Biosystems TaqMan miRNA assay, assay ID 002299
miR-103 miRNA assay kit Primer Applied Biosystems TaqMan miRNA assay, assay ID 000439
Penicillin-Streptomycin solution (100×) Reagent Sigma–Aldrich P4333 Original brand not specified
Insulin Reagent Sigma–Aldrich I-6634
FcR blocking reagent Reagent Miltenyi Biotec 120-000-442
FBS Reagent Sigma–Aldrich F2442 Replaces Benchmark Cat. #100-106
7-AAD Reagent Molecular Probes A-1310
M-MLV reverse transcriptase Reagent Invitrogen AM2044
Insulin Reagent Sigma–Aldrich I-6634
Bovine serum albumin (BSA) Reagent Sigma–Aldrich 05470 Original brand not specified
Platinum Pfx DNA polymerase Reagents Invitrogen 11708013
10 mM sNTP mix Reagent Invitrogen 18427-013 Original brand not specified
Dulbecco's phosphate buffered saline (PBS); without Ca2+ and Mg2+ Reagent Sigma–Aldrich D8537 Original brand not specified