FITC-conjugated monoclonal anti-CD44 (clone G44-26) |
Antibody |
BD Pharmingen |
555478 |
– |
FITC-mouse IgG2b isotype control immunoglobulin |
Antibody |
BD Pharmingen |
555742 |
– |
MX3005P Real-time PCR system |
Equipment |
Stratagene |
– |
Replaces ABI Prism 7900 SDS |
IMDM with GlutaMAX, HEPES and sodium pyruvate |
Medium |
Gibco/Life Technologies |
31980-030 |
This reagent was referred to as ‘DMEM’ in the original study |
miR-34a miRNA assay kit |
Primer |
Applied Biosystems |
TaqMan miRNA assay, assay ID 000426 |
– |
hsa-let-7b miRNA assay kit |
Primer |
Applied Biosystems |
TaqMan miRNA assay, assay ID 000378 |
– |
miR-191 miRNA assay kit |
Primer |
Applied Biosystems |
TaqMan miRNA assay, assay ID 002299 |
– |
miR-103 miRNA assay kit |
Primer |
Applied Biosystems |
TaqMan miRNA assay, assay ID 000439 |
– |
Penicillin-Streptomycin solution (100×) |
Reagent |
Sigma–Aldrich |
P4333 |
Original brand not specified |
Insulin |
Reagent |
Sigma–Aldrich |
I-6634 |
– |
FcR blocking reagent |
Reagent |
Miltenyi Biotec |
120-000-442 |
– |
FBS |
Reagent |
Sigma–Aldrich |
F2442 |
Replaces Benchmark Cat. #100-106 |
7-AAD |
Reagent |
Molecular Probes |
A-1310 |
– |
M-MLV reverse transcriptase |
Reagent |
Invitrogen |
AM2044 |
– |
Insulin |
Reagent |
Sigma–Aldrich |
I-6634 |
– |
Bovine serum albumin (BSA) |
Reagent |
Sigma–Aldrich |
05470 |
Original brand not specified |
Platinum Pfx DNA polymerase |
Reagents |
Invitrogen |
11708013 |
– |
10 mM sNTP mix |
Reagent |
Invitrogen |
18427-013 |
Original brand not specified |
Dulbecco's phosphate buffered saline (PBS); without Ca2+ and Mg2+
|
Reagent |
Sigma–Aldrich |
D8537 |
Original brand not specified |