Reagent	Type	Manufacturer	Catalog #	Comments
HEK293T cells	Cells	ATCC	#CRL-3216	Replaces 293FT cells (Clontech, R700-07) originally used
HT1080 cells	Cells	ATCC	#CCL-121	–
LAPC4 single cell suspension	Cells	From Protocol 2
ultra-fine II insulin syringe, ½ cc, 31G needle	Material	BD	328468	–
15 cm tissue culture dish	Materials	Corning (Sigma–Aldrich)	CLS430599	Original brand not specified
0.45 µm syringe filters	Materials	EMD Millipore	SLHP033RS	Original brand not specified
6 well tissue culture plate	Materials	Corning (Sigma–Aldrich)	CLS3516	Original brand not specified
IMDM with GlutaMAX, HEPES and sodium pyruvate	Medium	Gibco/Life Technologies	31,980-030	This reagent was referred to as ‘DMEM’ in the original study
IMDM with HEPES and sodium pyruvate	Medium	GIBCO/Life Technologies	12440053	–
EMEM	Medium	ATCC	30-2003	Original culture conditions for HT1080 cells unspecified
IMDM with GlutaMAX, HEPES and sodium pyruvate	Medium	Gibco/Life Technologies	31,980-030	This reagent was referred to as ‘DMEM’ in the original study
NOD/SCID mice, male, 6–8 weeks old	Mice	Jackson Laboratories	001303	–
REV packaging plasmid	Plasmid	Provided by original authors
RRE packaging plasmid	Plasmid	Provided by original authors
VSVG packaging plasmid	Plasmid	Provided by original authors
Lenti-ctl plasmid	Plasmid	System Biosciences	PMIRH000PA-1	–
Lenti-miR-34 plasmid	Plasmid	System Biosciences	PMIRH34aPA-1	–
GenElute endotoxin-free plasmid maxiprep kit	Reagent	Sigma–Aldrich	PLEX15-1KT	This kit replaces the Qiagen endo-free maxiprep kit used by the original authors
Lipofectamine 2000	Reagent	Invitrogen/Life Technologies	11,668-019	–
FBS	Reagent	Sigma–Aldrich	F2442	Replaces Benchmark Cat. #100-106
Matrigel, phenol red-free	Reagent	Corning	356237	Same item as BD Cat. #CB-40234C
Penicillin-Streptomycin solution (100×)	Reagent	Sigma–Aldrich	P4333	Original brand not specified
Fluorescent microscope	Equipment	Make and model to be recorded later		Original unspecified
Ultra-low attachment T75 tissue culture flasks	Materials	Corning	3814	Original unspecified
Dissecting microscope	Equipment	Make and model to be recorded later		Original unspecified