Reagent	Type	Manufacturer	Catalog #	Comments
Rabbit monoclonal α CD44 antibody	Antibody	AbCam	ab51037	–
Mouse monoclonal IgG2b α beta-actin	Antibody	Cell Signaling Technology	3700S	Original brand not specified
IRDye 800CW goat anti-rabbit IgG(H + L)	Antibody	LiCor	926-32211	–
IRDye 680LT goat anti-mouse IgG(H + L)	Antibody	LiCor	926-68020	–
BCA protein assay	Kit	Pierce	23227	–
PVDF membrane	Material	Millipore	IPVH00010	–
NuPAGE™ 4–12% Bis-Tris gels 1.0 mm, 10 well	Material	Invitrogen	NP0005	–
RIPA buffer	Reagent	Pierce	89,901	–
Phosphatase inhibitor cocktail II	Reagent	Sigma–Aldrich	P5726	–
Phosphatase inhibitor cocktail III	Reagent	Sigma–Aldrich	P0044	–
Complete-EDTA free tablet	Reagent	Roche	05892791001	–
NuPAGE™ MOPS SDS running buffer (20×)	Reagent	Invitrogen	NP0005	–
NuPAGE™ LDS sample buffer (4×)	Reagent	Invitrogen	NP0007	–
Sample reducing agent	Reagent	Invitrogen	NP0009	–
NuPAGE transfer buffer (20×)	Reagent	Invitrogen	NP006-1	–
Prestained protein marker	Reagent	Fermentas	SM0671	–
Ethanol	Reagent	Sinopharm Chemical Reagent	10009218	–
Sodium chloride (NaCl)	Reagent	Sinopharm Chemical Reagent	10019318	–
Tween-20	Reagent	Sigma–Aldrich	P1379	–
Tris buffered saline (TBS); 10× solution	Reagent	Sigma–Aldrich	T5912	Original brand not specified
Non-fat powder milk	Reagent	Sangon	NB0669-250g	–
Antibody dilute reagent solution	Reagent	Invitrogen	00-3218	–