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. 2015 Jul 31;5:12671. doi: 10.1038/srep12671

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FACS analysis was carried out to assess sequestration of (a) CD3⁺/CD8⁺ cells (b) CXCR3⁺/CD3⁺/CD8⁺ cells and (c) CD3⁺/CD8⁺/IFNγ⁺ in brain leukocytes. The experiment was repeated twice and two animals were used in each group.