Figure 3. The 54 full-length cytochrome P450 genes predicted in the Hypothenemus hampei genome clustered with Drosophila melanogaster genes representing the four insect clades: CYP2, CYP3, CYP4, and mitochondrial.

Predicted proteins in the cytochrome P450 family were identified by HMMER3 search with the Pfam CYP consensus model (PF00135.23). Evolutionary analyses were conducted using the Maximum Likelihood method in MEGA6. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (100 replicates) are shown next to the branches. Branches with less than 50% bootstrap support are condensed.