Figure 4. PPARγ modulation of LAT1 and TAUT expression requires de novo protein synthesis while it directly stimulates LAT2 gene transcription.

(A–C) the effect of protein inhibitor CHX on PPARγ-induced alternation of LAT1 (A) LAT2 (B) and TAUT (C) mRNA. Trophoblasts were pretreated with CHX (10⁻⁵ M) for 1 h, then treated with rosiglitazone (10⁻⁶ M) for 24 h. The level of LAT1, LAT2 and TAUT mRNA was assessed by real-time RT-PCR. Data are presented as mean percent control ± SEM of four cultures (n = 4) performed in triplicate. Ros, rosiglitazone. **P < 0.01 vs vehicle. &P < 0.05 vs Ros. (D) the effect of rosiglitazone on LAT2 promoter activity. Placental cells were transfected with LAT2 reporter plasmid and then treated with rosiglitazone (10⁻⁶ M) in the absence or presence of GW9662 (10⁻⁶ M) for 24 h. Values represent the mean ± SEM (n = 4). Ros, rosiglitazone. *P < 0.05 vs vehicle; &P < 0.05 vs Ros.