Figure 1.

Identification of Wnt/βcatenin target genes in adrenocortical cancer. (a) To identify gene's expression that is closely correlated with AXIN2 expression and that might also be Wnt/β-catenin targets, we performed Pearson's r correlation tests with expression data sets from two independent cohorts of ACC (cohort 1⁸ and cohort 2¹⁰). Each dot in left panels represents the Pearson correlation coefficient (r) for each gene in the two cohorts (detailed in figure S01-A and Supplementary Table S1). The genes best correlated with AXIN2 are represented in red (positively; r>0.6 in both cohorts) or blue (negatively; r<–0.6 in both cohorts). (b) The volcano plot in the right panels shows the differential expression of genes in three stable clones of the human adrenocortical cell line H295R, which carries a construct encoding a doxycyclin-inducible shRNA targeting CTNNB1 mRNA (shβ). Dots in gray represent genes that did not show significant changes in expression, dots in red on the left indicate the genes with significantly downregulated expression (adjust P-value<0.05 and log2 ratio<–1) and dots in blue on the right indicate the genes with significantly upregulated expression (adjust P-value<0.05 and log2 ratio>1). Black circles represent the genes common to both analyses.