Figure 6.

AFF3 silencing alters apoptosis, proliferation and colony forming. (a) Histogram of AFF3 mRNA levels and western blotting showing protein accumulation in Ctr and shAFF3 clones 5 days after addition of doxycyclin (dox) to the culture medium (0.2 mg/ml). (b) In the apoptosis illustration, histograms represent caspase-3/7 activity, after vehicle or dox treatment for 5 days, without or with staurosporin co-treatment for the last 6 h (50 ng/ml). (c) Cell survival, reported in graph labeled proliferation, was assessed by the MTT assay without or with dox (0.2 mg/ml) treatment for 1, 6, 12 or 16 days. (d) Colony formation by Ctr and shAFF3 clones without or with dox (0.2 mg/ml) treatment for 30 days was visualized by crystal-violet staining.