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. Author manuscript; available in PMC: 2016 Mar 19.
Published in final edited form as: Mol Cell. 2015 Mar 19;57(6):1059–1073. doi: 10.1016/j.molcel.2015.01.039

Figure 5. Reinitiation by 40S subunits following ABCE1-mediated splitting of post-TCs on model RHDV mRNA. Recycling and reinitiation activities of Ligatin and MCT1-DENR.

Figure 5

(A–C) Toe-printing analysis of factor requirements for reinitiation by 40S subunits following ABCE1-mediated splitting of post-TCs on (A, B) wt and (C) motifs 1/2* mutant RHDV mRNAs. (D–F) Toe-printing analysis of reinitiation by Ligatin and MCT1/DENR on (D, F) wt and (E) motifs 1/2* mutant RHDV mRNAs. (A–F) The positions of pre/post-TCs on ORF1 (black brackets) and 48S complexes on ORF2 AUG (red arrows) are shown on the right. Stop (black) and restart (red) codons are marked on the left. Free [Mg2+] is indicated on each panel.