Table 1. Comparing the design differences between the CUHK-HL V1 and the SureSelect 50Mb target enriched kits.

Target enrichment kit	CUHK-HL V1	SureSelect 50 Mb
Target region length (bp)	2 062 107	51 646 629
Number of baits	52 049	556 569
Baits layout	Overlapping baits, fourfold tiling across the target intervals	Intermediately adjacent, head-to-tail anchored baits across the target intervals
Targeted gene groups	78 known HL genes+174 candidates+entire mtDNA	GENCODE coding genes+noncoding RNAs from miRBase and Rfam
Targeted regions for each gene	All exons and UTRs+50 bp flanking sequences	All coding exons+10 bp flanking sequences
Proportion of the NSHL gene regionsa covered by the designed targets	0.992	0.942

Abbreviations: bp, base pair; UTR, un-translated regions.

^aGene regions are defined as all coding exons plus 10 bp flanking sequences at intron–exon junctions.