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. 2015 Jul 31;13:81. doi: 10.1186/s12958-015-0081-5

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© Diot et al. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — AdipoR1 (a), AdipoR2 (b), CMKLR1 (c), CCRL2 (d), and GPR1 (e), mRNA expression in theca and granulosa cells from F3/4 and F1 hierarchical follicles. Relative mRNA expression of adiponectin receptors (AdipoR1 and AdipoR2) and Chem receptors (CMKLR1, CCRL2 and GPR1) in theca and granulosa cells from turkey F3/4 and F1 hierarchical follicles, based on real-time RT-PCR. AdipoR1, AdipoR2, CMKLR1, CCRL2 and GPR1 mRNA expressions were measured relative to the geometric mean of the expression of three reference genes as described in Materials and Methods. Results are means ± SEM from six different animals in each follicular stage. Different capital letters indicate a significant effect of the type of cells (Theca vs Granulosa cells) whereas lower case letters indicate a significant effect of the follicular stage (F3/4 vs F1)