Fig. 7.

Plasma ADP concentration as determined by ELISA and immunoblot in turkey during a laying cycle. a. Plasma total ADP levels in turkey at different stages of laying [start (S:32 week-old), peak (P: 35 week-old), end (E: 55 week-old)] based on ELISA. All serums samples used in Western blotting in denaturing conditions (b) contained equal amounts of protein; this was confirmed by staining the nitrocellulose membrane with Ponceau. Blots were quantified using an arbitrary scale. The letters above each bar indicate significant differences (p < 0.05). Data are represented as mean ± SEM. (n = 24/ stage of laying period). c. Similar experiments were performed in b. in non denaturing conditions. The letters above each bar indicate significant differences (p < 0.05). Data are represented as mean ± SEM. (n = 24/ stage of laying period)