Skip to main content
. 2015 Jun 12;6(8):5006–5015. doi: 10.1039/c5sc01238d

Table 2. Summary of dsDNA-recognition characteristics of selected Invader probes a .

Invader X-modified Invader
 Y-modified Invader
Rec200 (%) C 50 (μM) t 50 (min) k obs (min–1) k rel Rec200 (%) C 50 (μM) t 50 (min) k obs (min–1) k rel
M1:M2 46 9.4 ND 1.1 × 10–3 1 51 3.9 498 1.4 × 10–3 1.3
 
M9:M10 74 3.4 185 7.5 × 10–3 6.8 92 0.9 211 4.3 × 10–3 3.9
 
M19:M20 >95 0.4 7 9.7 × 10–2 88 >95 0.3 47 1.5 × 10–2 13.5

aRec200 denotes degree of recognition when using Invader at a 200-fold molar excess. C 50, t 50 and k obs values obtained from Fig. 4, 5, and S7, respectively. k rel are calculated relative to the pseudo-first order rate constant for X1:X2.