Table 3. T _m and ΔG310rec values of Invader probes used in the nd-FISH study ^a .

Invader	Sequence	T m [ΔT m] (°C)				ΔG310rec (kJ mol–1)
		5′-Inv:cDNA	3′-Inv:cDNA	Invader	dsDNA target
INV1	5′-Cy3-AGCCCUGUGCCCTG	69.5 [+9.0]	74.5 [+14.0]	58.0 [–2.5]	60.5	–46
	3′-TCGGGACACGGGAC-Cy3
INV2	5′-Cy3-CCUGUGCCCTG	59.5 [+9.0]	65.5 [+15.0]	48.0 [–2.5]	50.5	–30
	3′-GGACACGGGAC-Cy3
INV3	5′-Cy3-CCUGTGCCCTG	59.0 [+8.5]	64.0 [+13.5]	47.0 [–3.5]	50.5	–24
	3′-GGACACGGGAC-Cy3
INV4	5′-Cy3-AGCCCUGTGCCCTG	69.5 [+9.0]	75.5 [+15.0]	61.5 [+1.0]	60.5	–29
	3′-TCGGGACACGGGAC-Cy3

^aΔT _m = change in T _m values relative to corresponding unmodified and unlabeled reference duplex. For experimental details see Table 1. A, C and U denote 2′-O-(pyren-1-yl)methyladenosine,⁴⁷ 2′-O-(pyren-1-yl)methylcytidine⁴⁷ and 2′-O-(pyren-1-yl)methyluridine (monomer X), respectively.