Fig. 1.

SDS-PAGE analysis of ARG I secreted in the cell culture supernatant. M protein molecular weight marker (the molecular weight of each band is indicated on left); a Shake-flash culture supernatants, lane 1–6, culture supernatants collected from 1 to 6 days, lane 7, negative control (P. pastoris bearing the pHBM905A vector); b High-density fermentation supernatants, lane 1–7, fermentation supernatants collected from 12 to 84 h; c Purified enzyme, lane 1, the protein purified with ultrafiltration, lane 2, the protein purified with Ni²⁺-affinity chromatography; d Western blot of ARG I, lane 1, negative control (P. pastoris bearing the pHBM905A vector), lane 2, positive control (Lipase from Proteus bacillus with a 6×His--tag), lane 3, the protein purified with Ni²⁺-affinity chromatography, lane 4, the protein purified with ultrafiltration