Table 1.
Strains and plasmids used in this study
| Strains or plasmids | Genotype or relevant characteristics | Source or reference |
|---|---|---|
| Strain | ||
| E. coli DH5α | Invitrogen | |
| E. aerogenes KCTC 2190 | Wild type | Korean Collection for Type Culture |
| EMY-01 | E. aerogenes KCTC 2190 △ldhA | [27] |
| EMY-68 | E. aerogenes KCTC 2190 △ldhA △scrR | [19] |
| EMY-69 | E. aerogenes KCTC 2190 △ldhA △cra | This study |
| EMY-70 | E. aerogenes KCTC 2190 △ldhA △scrR △cra | This study |
| EMY-70S | Plasmid-based scrAB overexpression strain of EMY-70 by introduction of pZS21MCS::scrAB | This study |
| EMY-70SP | Plasmid-based ptsG overexpression strain of EMY-70S by introduction of pZA31MCS::ptsG | This study |
| Plasmid | ||
| pKM208 | lacI, λ Red + Gam-producing vector, tac_promoter, f1_ori, Amp R | Addgene |
| pCP20 | FLP recombinase-producing vector, cI857, pSC101 ori, Amp R, Cm R | [40] |
| pKD4 | FRT flanked resistance cassette involved vector, oriRγ, Km R | [40] |
| pZA31MCS | E. coli—K. pneumoniae shuttle vector, PLtetO-1, p15A ori, CmR | Expressys |
| pZS21MCS | E. coli—K. pneumoniae shuttle vector, PLtetO-1, pSC101 ori, Km R | Expressys |
| pZA31MCS::ptsG | pZA31MCS derivative containing ptsG, PLtetO-1, p15A ori, CmR | This study |
| pZS21MCS::scrAB | pZS21MCS derivative containing scrAB, PLtetO-1, pSC101 ori, Km R | This study |