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. Author manuscript; available in PMC: 2015 Jul 31.
Published in final edited form as: J Am Chem Soc. 2015 May 15;137(20):6432–6435. doi: 10.1021/jacs.5b00275

Figure 2.

Figure 2

The yuaA-Spinach2 biosensor detects altered cyclic di-AMP levels in Listeria monocytogenes.(a) Fluorescence microscopy images of L. monocytogenes 10403s strains expressing yuaA-Spinach2 tRNAs after incubation with the membrane dye FM4-64 or DFHBI (left and middle panels, respectively; right panels show merge). (b) Flow cytometry analysis of L. monocytogenes strains expressing yuaA-Spinach2 tRNAs. Error bars represent standard deviation between four independent biological replicates. (c) Flow cytometry analysis of WT or ∆pdeA strains with no complementation or complemented with different pdeA enzyme constructs. The domain arrangement for PdeA is shown; the transmembrane (TM) domain was not included in the enzyme constructs. Error bars represent standard deviation between three independent biological replicates.