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. 2015 Jul 31;11(7):e1005412. doi: 10.1371/journal.pgen.1005412

Fig 3. The repressor domain functions as an independent regulatory module.

Fig 3

(A) The ems0.35 enhancer (blue) drives expression to the posterior spiracles (B). (C) Expression of ems0.35-Z is maintained in Df(1)os1A mutant embryos lacking JAK/STAT pathway activity. (D) Fusion of the crb STAT-binding and repressor modules (red and green) to ems0.35 (crb313-ems0.35) does not affect spiracle expression in wild type embryos (E), but abolishes spiracle expression in Df(1)os1A embryos (F). (G) When the STAT-binding sites are mutated in this construct (crb313 MUT-ems0.35) spiracle activity is lost (H). Note that the crb313-ems0.35 fusion construct generates a novel gut pattern of expression that is STAT independent as it is unaffected in Df(1)os1A mutants or when the STAT sites are mutated. Arrowheads: posterior spiracle primordium.